Journal: Applied and Environmental Microbiology

Article Title: Improved Enumeration of Lactic Acid Bacteria in Mesophilic Dairy Starter Cultures by Using Multiplex Quantitative Real-Time PCR and Flow Cytometry-Fluorescence In Situ Hybridization

doi: 10.1128/aem.02283-05

Figure Lengend Snippet: FIG. 2. Resolution of the multiplex qPCR assay, shown as CT val- ues versus DNA template concentrations in the L. lactis subsp. lactis (black bars), L. lactis subsp. cremoris (light gray bars), and Leuconostoc (dark gray bars) channels. DNA extracted from PROBAT with the DNeasy tissue kit was diluted 1:100 to obtain the starting concentra- tion (100%). This was then further diluted at increasingly narrower steps, 2-, 1.7-, 1.5-, 1.3-, and 1.1-fold. Except for the pairwise compar- ison between the CT values of the 1.3- versus the 1.1-fold dilution, values were significantly different (analysis of variance; P 0.05). Error bars indicate standard deviations of CT values of four replicates.

Article Snippet: Defined PROBAT-like cultures were created by mixing member strains of the three taxa Leuconostoc mesenteroides subsp. cremoris (DSMZ 20346T and DGCC 8), Lactococcus lactis subsp. lactis (DGCC strains 113, 133, 456, 1212, and 1306), and L. lactis subsp. cremoris (DGCC strains 16, 111, 453, 563, and 1224).

Techniques: Multiplex Assay

Journal: Applied and Environmental Microbiology

Article Title: Improved Enumeration of Lactic Acid Bacteria in Mesophilic Dairy Starter Cultures by Using Multiplex Quantitative Real-Time PCR and Flow Cytometry-Fluorescence In Situ Hybridization

doi: 10.1128/aem.02283-05

Figure Lengend Snippet: FIG. 3. Predicted compositions of eight defined PROBAT-like cul- tures (A through M) calculated from the percentages of CFU of blends of strains of the taxa. Black bars, Lactococcus lactis subsp. lactis; light gray bars, L. lactis subsp. cremoris; dark gray bars, L. mesenteroides subsp. cremoris. Data shown are mean percentages and standard de- viations (error bars) of two replicate counts on triple sugar tryptone and MRS agar for lactococci and leuconostoc cells, respectively.

Article Snippet: Defined PROBAT-like cultures were created by mixing member strains of the three taxa Leuconostoc mesenteroides subsp. cremoris (DSMZ 20346T and DGCC 8), Lactococcus lactis subsp. lactis (DGCC strains 113, 133, 456, 1212, and 1306), and L. lactis subsp. cremoris (DGCC strains 16, 111, 453, 563, and 1224).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Improved Enumeration of Lactic Acid Bacteria in Mesophilic Dairy Starter Cultures by Using Multiplex Quantitative Real-Time PCR and Flow Cytometry-Fluorescence In Situ Hybridization

doi: 10.1128/aem.02283-05

Figure Lengend Snippet: FIG. 4. Relative proportions (percentages) of the taxa Lactococcus lactis subsp. lactis (black bars), L. lactis subsp. cremoris (light gray bars), and Leuconostoc mesenteroides subsp. cremoris (dark gray bars) in eight defined PROBAT-like cultures. (A to C) Estimated relative proportions based on qPCR of DNA isolated with the DNeasy tissue (QIAGEN), FastDNA spin (Qbiogene), and Bilatest Bac (Bilatec) kits, respectively. (D) Values determined by FLOW-FISH. Error bars indicate standard deviations of three and two replicates for qPCR and FLOW-FISH, respectively.

Article Snippet: Defined PROBAT-like cultures were created by mixing member strains of the three taxa Leuconostoc mesenteroides subsp. cremoris (DSMZ 20346T and DGCC 8), Lactococcus lactis subsp. lactis (DGCC strains 113, 133, 456, 1212, and 1306), and L. lactis subsp. cremoris (DGCC strains 16, 111, 453, 563, and 1224).

Techniques: Isolation

Journal: International journal of systematic and evolutionary microbiology

Article Title: Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China.

doi: 10.1099/ijs.0.64968-0

Figure Lengend Snippet: Fig. 2. Phylogenetic positions based on 16S rRNA gene sequences of strain JBT and the type strains of species of the genus Brachybacterium. Dermabacter hominis was included as an outgroup. Bootstrap values above 50 % (expressed as percen- tages of 100 replications) are indicated at branch points. Bar, 2 % sequence divergence.

Article Snippet: The reference strains used were: Brachybacterium rhamnosum LMG 19848T, Brachybacterium fresconis LMG 20336T, Brachybacterium nesterenkovii LMG 19549T (5DSM 9573T), Brachybacterium paraconglomeratum LMG 19861T and Brachybacterium sacelli LMG 20345T, all obtained from the Laboratorium voor Microbiologie, Rijksuniversiteit Gent, Ledeganckstraat 35, B-9000, Gent, Belgium; and Brachybacterium muris DSM 15460T, which was obtained from the DSMZ, Braunschweig, Germany.

Techniques: Sequencing